| # | Swimmer | Team | Seed | Time | Diff |
| 2 |
Wintsch, Layla A (10)
|
VADC |
47.01 |
42.84 |
-4.17 |
| 3 |
Hofer, Jennifer R (8)
|
VASEVA |
50.24 |
43.31 |
-6.93 |
| 4 |
Tillery, Paige E (10)
|
VATIDE |
44.69 |
45.42 |
0.73 |
| 5 |
Claudy, Julia A (9)
|
NCWA |
53.90 |
48.02 |
-5.88 |
| 6 |
Ayala-Irizarry, Alondra K (10)
|
VASEVA |
50.63 |
48.66 |
-1.97 |
| 7 |
Shaltayeva, Victoria (9)
|
VASEVA |
55.39 |
49.89 |
-5.50 |
| 8 |
Owens, Jada S (9)
|
VASEVA |
51.61 |
50.16 |
-1.45 |
| 9 |
Barber, Lucy W (9)
|
VACGBD |
56.36 |
51.99 |
-4.37 |
| 10 |
Nance, Madelyn P (8)
|
VASEVA |
59.49 |
58.40 |
-1.09 |
| 11 |
Jin, Gloria (9)
|
MDMSC |
1:08.53 |
1:02.91 |
-5.62 |
| 12 |
Maciejewski, Mckinley K (7)
|
VACGBD |
1:02.69 |
1:05.23 |
2.54 |
|
Wells, Kenley A (9)
|
VADC |
1:02.97 |
DQ |
- |
|
Walters, Alanna E (10)
|
VAYOBX |
2:20.00 |
NS |
- |
|
Jimenez, Saniya N (9)
|
VASEVA |
1:20.53 |
NS |
- |